J Peking Univ[Health Sci],2002,34:676-679

Abnormal expression of a novel Apoptosis-promoting molecule TFAR19（PDCD5） in the bone marrow cells from adult chronic myeloid leukemia

RUAN Guo-Rui, CHEN Shan-Shan, CHANG Yan, FU Jia-Yu, WAN Hui, Qin Ya-Zhen, LI Jin-Lan, LIU Yan-Rong,（Institute of Hematology , Peking University People`Hospital, Beijing 100044,China）

KEY WORDS Leukemia, myeloid chronic ; Apoptosis ; TFAR19 ; PDCD5 ; Bone marrow cells

SUMMARY Objective: To estimate a novel apoptosis-promoting molecule TFAR19 expression in the bone marrow cells from adult chronic myelogenous leukemia (CML) for its significance in the pathogenesis and disease progression of CML. Methods: Flow cytometry （FCM）assay was used for detection of TFAR19 expression in different groups of cells from normal and CML patient bone marrows by 15 monoclonal antibodies with different fluorescent markers. Results: The mean TFAR19 fluorescence intensity was significantly lower in marrow nucleated cells（MNC）from the marrow of 20 untreated CML-CP（chronic phase, CP） and 13 CML-AP/BP（accelerated or blastic phase, AP/BP）patients than that from the marrow of 10 normal subjects（5 793 1 536 vs.7 260.9 781, p 0.01; 4 293 1 082 vs. 7 260 781, p 0.01, respectively）. TFAR19 fluorescence intensity was lower in the marrow granulocytes from CML-CP and CML-AP\BP patients than that from normal（6 240 1 734 vs 8 317 726，p 0.01;5 759 1 1487，8 317 726，p 0.01, respectively）.Furthermore, the TFAR19 in the marrow lymphocytes from CML-CP and CML-AP/BP patients it was also lower than that from the normal.（1 292 639 vs 2 271 820，p 0.01;1 231 281，2 271 820，p 0.01, respectively,）In addition , in the marrow nucleated cells from CML-AP/BP patients it was further lower than that from CML-CP patients(4 293 1 082 vs 5 793 1 536，p 0.01). Conclusions: It can be concluded that TFAR19 expression in marrow nucleated cells was lower in CML patients versus normal. TFAR19 expression was lower in the marrow granulocytes with CML-CP and CML-AP/BP patients versus the normal. It suggests that the abnormality of TFAR19 expression may play an important role in the evolution of CML.

中文摘要